Biomolecular Engineering

Directed evolution and bioprocesses with industrial importance

Learn from nature, go beyond nature

To contribute to human health and sustainable society, the Biomolecular Engineering Lab is devoted to research and education on novel bioprocesses as well as yeast-based high-throughput analysis systems for genes whose biological functions are not fully understood.

1. Directed evolution of proteins for industrial/diagnostic applications
Directed evolution is a methodology used in protein engineering, in which proteins or microbes are randomly diversified and subjected to functional screening to "breed" mutant strains with desirable properties. Novel methods for efficient mutagenesis and screening have also been developed in our lab.
2. Interaction targeting
Interactions between proteins play crucial roles in every biological process as well as disease development and pathogen infection. Peptide or protein fragments that can specifically interfere with those unfavorable interactions will provide insight into novel avenues of drug discovery.
3. Yeast extracellular production of recombinant proteins
Extracellular expression facilitates a cost-effective means of producing industrially useful proteins. However, depending on the protein to be expressed, the production results in a poor yield, which is occasionally accompanied with a loss of the expression plasmid and thus hampered growth of the host. We are currently analyzing the molecular mechanism behind this low productivity.


Yasuaki KawarasakiPhD


Mizuki TanakaPhD

Details are here



Based on recombinant DNA technologies, yeast molecular biology, and protein expression systems, novel applications as well as analytical methods have been established in our laboratory. Our research focuses on (i) disease-related protein-protein interactions, (ii) membrane proteins which are responsible for taste sensing, and (iii) the directed evolution of industrially useful enzymes including laccases. See our web pages at for detailed information.


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